RANK, SAP-Exo + Seq, parents

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Date

2020-12-14

Last modified: Thu May 20 20:06:02 UTC 2021

Objective

Last modified: Thu May 20 20:06:02 UTC 2021

Method

<p><strong>SAP-Exo Clean-up</strong></p> 
<p>PCR templates: <a id="29797" class="linkedRecord mceNonEditable" href="/globalId/SD29797" data-globalid="SD29797" data-name="RANK E9 PCR UK20-03, UK20-04">SD29797: RANK E9 PCR UK20-03, UK20-04</a></p> 
<div class="rsCalcTableDiv mceNonEditable" data-tabledata="{&quot;data&quot;:[[null,&quot;&quot;,&quot;Number of rxns&quot;,&quot;4&quot;,null,null,null,null,null,null,null,null],[null,null,null,null,null,null,null,null,null,null,null,null],[null,null,&quot;per rxn [µL]&quot;,&quot;total volume [µL]&quot;,null,null,null,null,null,null,null,null],[null,&quot;Mill. water&quot;,&quot;1.625&quot;,&quot;=D1*C4&quot;,null,null,null,null,null,null,null,null],[null,&quot;Exonuclease I&quot;,&quot;0.075&quot;,&quot;=D1*C5&quot;,null,null,null,null,null,null,null,null],[null,&quot;tSAP&quot;,&quot;0.3&quot;,&quot;=D1*C6&quot;,null,null,null,null,null,null,null,null],[null,null,null,null,null,null,null,null,null,null,null,null],[null,&quot;Total&quot;,&quot;2&quot;,null,null,null,null,null,null,null,null,null],[null,null,&quot;+ 8 µL PCR&quot;,null,null,null,null,null,null,null,null,null]],&quot;settings&quot;:&quot;dummy&quot;}"> 
 <table class="rsCalcTable"> 
  <thead> 
   <tr> 
    <th>&nbsp;</th> 
    <th scope="col">A</th> 
    <th scope="col">B</th> 
    <th scope="col">C</th> 
    <th scope="col">D</th> 
    <th scope="col">E</th> 
    <th scope="col">F</th> 
    <th scope="col">G</th> 
    <th scope="col">H</th> 
    <th scope="col">I</th> 
    <th scope="col">J</th> 
    <th scope="col">K</th> 
    <th scope="col">L</th> 
   </tr> 
  </thead> 
  <tbody> 
   <tr> 
    <th scope="row">1</th> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>Number of rxns</td> 
    <td>4</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
   </tr> 
   <tr> 
    <th scope="row">2</th> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
   </tr> 
   <tr> 
    <th scope="row">3</th> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>per rxn [µL]</td> 
    <td>total volume [µL]</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
   </tr> 
   <tr> 
    <th scope="row">4</th> 
    <td>&nbsp;</td> 
    <td>Mill. water</td> 
    <td>1.625</td> 
    <td>6.5</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
   </tr> 
   <tr> 
    <th scope="row">5</th> 
    <td>&nbsp;</td> 
    <td>Exonuclease I</td> 
    <td>0.075</td> 
    <td>0.3</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
   </tr> 
   <tr> 
    <th scope="row">6</th> 
    <td>&nbsp;</td> 
    <td>tSAP</td> 
    <td>0.3</td> 
    <td>1.2</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
   </tr> 
   <tr> 
    <th scope="row">7</th> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
   </tr> 
   <tr> 
    <th scope="row">8</th> 
    <td>&nbsp;</td> 
    <td>Total</td> 
    <td>2</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
   </tr> 
   <tr> 
    <th scope="row">9</th> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>+ 8 µL PCR</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
    <td>&nbsp;</td> 
   </tr> 
  </tbody> 
 </table> 
</div> 
<p>Cycler programme <strong>EXOSAP:</strong></p> 
<p>37 °C, 20 min</p> 
<p>85 °C, 15 min</p> 
<p>10 °C, 5 min</p> 
<p><strong>Sanger sequencing by Microsyth Seqlab</strong></p> 
<p>2 µL of 10 µM primer</p> 
<p>2 µL SAP-Exo PCR product</p> 
<p>11 µL water</p> 
<table style="border-collapse: collapse; width: 100%; height: 136px;" border="1" width="300" cellpadding="5"> 
 <tbody> 
  <tr style="height: 40px;"> 
   <td style="width: 20%; height: 40px;"> <p><strong>B</strong><strong>arcode</strong></p> </td> 
   <td style="width: 20%; height: 40px;"><strong>File name</strong></td> 
   <td style="width: 20%; height: 40px;"><strong>Template</strong></td> 
   <td style="width: 20%; height: 40px;"><strong>PCR/Target</strong></td> 
   <td style="width: 20%; height: 40px;"><strong>Primer name</strong></td> 
  </tr> 
  <tr style="height: 32px;"> 
   <td style="width: 20%; height: 32px;">91 683 451</td> 
   <td style="width: 20%; height: 32px;">UK20-03_RANK-E9_E9F_AFH</td> 
   <td style="width: 20%; height: 32px;">UK20-03, gDNA</td> 
   <td style="width: 20%; height: 32px;">RANK exon 9</td> 
   <td style="width: 20%; height: 32px;">RANK_e9f</td> 
  </tr> 
  <tr style="height: 32px;"> 
   <td style="width: 20%; height: 32px;">91 683 452</td> 
   <td style="width: 20%; height: 32px;">UK20-03_RANK-E9_E9R_AFH</td> 
   <td style="width: 20%; height: 32px;">UK20-03, gDNA</td> 
   <td style="width: 20%; height: 32px;">RANK exon 9</td> 
   <td style="width: 20%; height: 32px;">RANK_e9r</td> 
  </tr> 
  <tr style="height: 16px;"> 
   <td style="width: 20%; height: 16px;">91 683 453</td> 
   <td style="width: 20%; height: 16px;">UK20-04_RANK-E9_E9F_AFH</td> 
   <td style="width: 20%; height: 16px;">UK20-04, gDNA</td> 
   <td style="width: 20%; height: 16px;">RANK exon 9</td> 
   <td style="width: 20%; height: 16px;">RANK_e9f</td> 
  </tr> 
  <tr style="height: 16px;"> 
   <td style="width: 20%; height: 16px;">91 683 454</td> 
   <td style="width: 20%; height: 16px;">UK20-04_RANK-E9_E9R_AFH</td> 
   <td style="width: 20%; height: 16px;">UK20-04, gDNA</td> 
   <td style="width: 20%; height: 16px;">RANK exon 9</td> 
   <td style="width: 20%; height: 16px;">RANK_e9r</td> 
  </tr> 
 </tbody> 
</table> 
<p>&nbsp;</p> 
<p>&nbsp;</p>

Last modified: Thu May 20 20:06:02 UTC 2021

Results

<div class="attachmentDiv mceNonEditable"> <a href="/Streamfile/39012" target="_blank" rel="noopener"> <img class="attachmentIcon" src="/images/icons/unknownDocument.png?width=32&amp;height=32" width="32" height="32"> </a> 
 <p class="attachmentP"><a id="attachOnText_39012" class="attachmentLinked" href="/Streamfile/39012" target="_blank" rel="noopener" data-type="Miscellaneous">UK20-03_RANK-E9_E9F_AFH.ab1</a></p> 
 <div id="attachmentInfoDiv_39012" class="attachmentInfoDiv"> 
  <img class="attachmentInfoIcon" src="/images/getInfo12.png"> 
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<div class="attachmentDiv mceNonEditable"> <a href="/Streamfile/39013" target="_blank" rel="noopener"> <img class="attachmentIcon" src="/images/icons/unknownDocument.png?width=32&amp;height=32" width="32" height="32"> </a> 
 <p class="attachmentP"><a id="attachOnText_39013" class="attachmentLinked" href="/Streamfile/39013" target="_blank" rel="noopener" data-type="Miscellaneous">UK20-03_RANK-E9_E9R_AFH.ab1</a></p> 
 <div id="attachmentInfoDiv_39013" class="attachmentInfoDiv"> 
  <img class="attachmentInfoIcon" src="/images/getInfo12.png"> 
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<div class="attachmentDiv mceNonEditable"> <a href="/Streamfile/39014" target="_blank" rel="noopener"> <img class="attachmentIcon" src="/images/icons/unknownDocument.png?width=32&amp;height=32" width="32" height="32"> </a> 
 <p class="attachmentP"><a id="attachOnText_39014" class="attachmentLinked" href="/Streamfile/39014" target="_blank" rel="noopener" data-type="Miscellaneous">UK20-04_RANK-E9_E9F_AFH.ab1</a></p> 
 <div id="attachmentInfoDiv_39014" class="attachmentInfoDiv"> 
  <img class="attachmentInfoIcon" src="/images/getInfo12.png"> 
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<div class="attachmentDiv mceNonEditable"> <a href="/Streamfile/39015" target="_blank" rel="noopener"> <img class="attachmentIcon" src="/images/icons/unknownDocument.png?width=32&amp;height=32" width="32" height="32"> </a> 
 <p class="attachmentP"><a id="attachOnText_39015" class="attachmentLinked" href="/Streamfile/39015" target="_blank" rel="noopener" data-type="Miscellaneous">UK20-04_RANK-E9_E9R_AFH.ab1</a></p> 
 <div id="attachmentInfoDiv_39015" class="attachmentInfoDiv"> 
  <img class="attachmentInfoIcon" src="/images/getInfo12.png"> 
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</div> 
<div class="attachmentDiv mceNonEditable"> <a href="/Streamfile/39016" target="_blank" rel="noopener"> <img class="attachmentIcon" src="/images/icons/pdf.png?width=32&amp;height=32" width="32" height="32"> </a> 
 <p class="attachmentP"><a id="attachOnText_39016" class="attachmentLinked" href="/Streamfile/39016" target="_blank" rel="noopener" data-type="Documents">UK20-04_Vater_RANK_E9_AFH.pdf</a></p> 
 <div id="attachmentInfoDiv_39016" class="attachmentInfoDiv"> 
  <img class="attachmentInfoIcon" src="/images/getInfo12.png"> 
 </div> 
</div> 
<div class="attachmentDiv mceNonEditable"> <a href="/Streamfile/39017" target="_blank" rel="noopener"> <img class="attachmentIcon" src="/images/icons/pdf.png?width=32&amp;height=32" width="32" height="32"> </a> 
 <p class="attachmentP"><a id="attachOnText_39017" class="attachmentLinked" href="/Streamfile/39017" target="_blank" rel="noopener" data-type="Documents">UK20-03_Mutter_RANK_E9_AFH.pdf</a></p> 
 <div id="attachmentInfoDiv_39017" class="attachmentInfoDiv"> 
  <img class="attachmentInfoIcon" src="/images/getInfo12.png"> 
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</div> 
<p class="mceTmpParagraph">&nbsp;</p> 
<p><strong>Samples and results:</strong></p> 
<table style="width: 344px;" border="1" width="300" cellpadding="5"> 
 <tbody> 
  <tr> 
   <td style="width: 56.03125px;"> <p><strong>B</strong><strong>arcode</strong></p> </td> 
   <td style="width: 87.9375px;"><strong>File name</strong></td> 
   <td style="width: 60.4375px;"><strong>Template</strong></td> 
   <td style="width: 75.203125px;"><strong>PCR/Target</strong></td> 
   <td style="width: 66.921875px;"><strong>Primer name</strong></td> 
   <td style="width: 45.125px;"><strong>Seq. quality</strong></td> 
   <td style="width: 0px;"><strong>Result</strong></td> 
  </tr> 
  <tr> 
   <td style="width: 56.03125px;">91 683 451</td> 
   <td style="width: 87.9375px;">UK20-03_RANK-E9_E9F_AFH</td> 
   <td style="width: 60.4375px;">UK20-03, gDNA</td> 
   <td style="width: 75.203125px;">RANK exon 9</td> 
   <td style="width: 66.921875px;">RANK_e9f</td> 
   <td style="width: 45.125px;">good</td> 
   <td style="width: 0px;">carrier of c.1266delG (c.1268delA?)</td> 
  </tr> 
  <tr> 
   <td style="width: 56.03125px;">91 683 452</td> 
   <td style="width: 87.9375px;">UK20-03_RANK-E9_E9R_AFH</td> 
   <td style="width: 60.4375px;">UK20-03, gDNA</td> 
   <td style="width: 75.203125px;">RANK exon 9</td> 
   <td style="width: 66.921875px;">RANK_e9r</td> 
   <td style="width: 45.125px;">good</td> 
   <td style="width: 0px;">carrier of c.1266delG (c.1268delA?)</td> 
  </tr> 
  <tr> 
   <td style="width: 56.03125px;">91 683 453</td> 
   <td style="width: 87.9375px;">UK20-04_RANK-E9_E9F_AFH</td> 
   <td style="width: 60.4375px;">UK20-04, gDNA</td> 
   <td style="width: 75.203125px;">RANK exon 9</td> 
   <td style="width: 66.921875px;">RANK_e9f</td> 
   <td style="width: 45.125px;">good</td> 
   <td style="width: 0px;">carrier of c.1093G&gt;T</td> 
  </tr> 
  <tr> 
   <td style="width: 56.03125px;">91 683 454</td> 
   <td style="width: 87.9375px;">UK20-04_RANK-E9_E9R_AFH</td> 
   <td style="width: 60.4375px;">UK20-04, gDNA</td> 
   <td style="width: 75.203125px;">RANK exon 9</td> 
   <td style="width: 66.921875px;">RANK_e9r</td> 
   <td style="width: 45.125px;">good</td> 
   <td style="width: 0px;">carrier of c.1093G&gt;T</td> 
  </tr> 
 </tbody> 
</table>

Last modified: Thu May 20 20:06:02 UTC 2021

Conclusion

Last modified: Thu May 20 20:06:02 UTC 2021

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RANK, SAP-Exo + Seq, parents

contact: robert.day@icloud.com

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